Does ischemic preconditioning trigger translocation of protein kinase C in the canine model?
Authors:
Journal: Circulation
Publication Type: Journal Article
Date: 1995
DOI: 10.1161/01.cir.92.6.1546
ID: 7664439
Abstract
Brief episodes of ischemia protect or "precondition" the heart and reduce the size of infarcts caused by subsequent sustained coronary artery occlusion, yet the mechanisms responsible for this cardioprotection remain unresolved. We tested the theory that translocation of protein kinase C (PKC) to the myocyte membranes, initiated in response to brief preconditioning ischemia and manifest during the initial minutes of the sustained occlusion, mediates this phenomenon by attempting to (1) blunt the cardioprotective effects of preconditioning by administration of the PKC inhibitors H-7 and polymyxin B, (2) visualize by fluorescence staining and confocal microscopy changes in the amount or location of PKC, and (3) quantify by incorporation of 32P into PKC-specific peptide changes in the subcellular distribution of PKC in preconditioned versus control hearts.
Chemical List
- Isoquinolines|||Piperazines|||1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine|||Protein Kinase C|||Tetradecanoylphorbol Acetate