Cardiovascular Research

Avoidance of immune response prolongs expression of genes delivered to the adult rat myocardium by replication-defective adenovi

Authors: M J Quiñones|||J Leor|||R A Kloner|||M Ito|||M Patterson|||W F Witke|||L Kedes

Affiliations: + Expand

Abstract

Gene delivery is a rapidly expanding field with potential applications to every human organ system. Recently, adenoviruses have been used as efficient vectors for in vivo gene transfer into the myocardium. These methods, however, have shown a sharp decline of gene expression after 1 week. To test the hypothesis that an immune-effector mechanism is involved in this decline, we compared the results after injection of adenovirus-5 carrying the beta-galactosidase gene (Ad beta-gal) into the left ventricular myocardium of athymic nude rats (NDRs) versus immunocompetent Sprague-Dawley rats (SDRs).

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Chemical List

Collagen